A multiple-stage HEPA filter and pre-filter can be developed to ensure the laminar hood is filtering air particles efficiently and keeping Contact APC for Custom HEPA Filters for Laminar HoodsĪPC Filtration has been designing, testing, and manufacturing custom HEPA filters for laminar flow cabinet hoods. Often, laminar hoods will require custom HEPA filters to be created to ensure they create the right pressure drop and fit the equipment perfectly to prevent leaks. Air is taken through this HEPA filtration system and exhausted across the work surface as part of the laminar flow process.īecause a laminar flow cabinet is enclosed on the sides, a constant positive air pressure can be maintained to prevent the intrusion of contaminated room air and personnel contamination. This set up id designed to create a particle-free working environment and provide product protection from contamination. Laminar flow hoods create this unidirectional flow of air with one or more HEPA filters and pre-filters. Class III biosafety cabinets provide the highest attainable level of protection to personnel and the environment and are suitable for human pathogens and BSL-4 materials. Class II are designed for work involving BSL-1, 2, and 3 materials and provide an aseptic environment for cell culture experiments. Class I is suitable for laboratory personnel with similar air flow characteristics to chemical fume hoods. Each of these are suitable for working with different types of materials as each has its own level of protection and filtration. There are three classes of laminar flow hoods, Class I, II, and III. The flow can either be horizontal or vertical, depending on the lab’s needs. Laminar flow hoods work to protect the working environment from dust and other airborne contaminants by maintaining a consistent, constant, unidirectional flow of filtered air over the work area. They are a key part of any laboratory and a requirement for keeping samples free of contaminants that could damage them. The light must be switched off when the cabinet is being used, to limit exposure to skin and eyes as stray ultraviolet light emissions can cause cancer and cataracts.Laminar flow cabinets are used in a variety of environments, particularly where a clean air environment is required for smaller items or samples. Germicidal lamps are usually kept on for fifteen minutes to sterilize the interior before the cabinet is used. Laminar flow cabinets may have a UV-C germicidal lamp to sterilize the interior and contents before usage to prevent contamination of the experiment. Such hoods exist in both horizontal and vertical configurations, and there are many different types of cabinets with a variety of airflow patterns and acceptable uses. The cabinet is usually made of stainless steel with no gaps or joints where spores might collect. Due to the direction of air flow, the sample is protected from the user but the user is not protected from the sample. Air is drawn through a HEPA filter and blown in a very smooth, laminar flow towards the user. Preparation of microbiological samples in a laminar chamber.Ī laminar flow cabinet or tissue culture hood is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive materials.
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